Informasi ringkas dokumen repository.
| Advisor(s) | Sembiring, Bunga Mari | |
| Author(s) | NANDA, PUTRI TARI | |
| Issued Date | 2026 | |
| Abstract | Purple yam (Dioscorea alata L.) is one of the food crops that contains various secondary metabolites and has potential as a source of natural antioxidants. This study aimed to determine the antioxidant activity of the ethanol extract of purple yam (Dioscorea alata L.) using the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) method by UV-Visible spectrophotometry, determine its IC₅₀ value, and validate the analytical method. This study was an experimental laboratory study. Purple yam simplicia was extracted by maceration using 96% ethanol. Antioxidant activity was determined using the DPPH method with vitamin C as a reference standard and measured using a UV-Visible spectrophotometer at a wavelength of 517 nm. The test solution concentrations used were 4, 6, 8, 10, and 12 ppm. Phytochemical screening showed that the purple yam simplicia contained alkaloids, flavonoids, tannins, saponins, and steroids. The results of the antioxidant activity test showed that the ethanol extract of purple yam had antioxidant activity with an IC₅₀ value of 16.94 ppm, while vitamin C had an IC₅₀ value of 9.26 ppm. The linear regression equation of the purple yam ethanol extract was Y = 3.030x − 1.3133. Method validation results showed a linearity correlation coefficient (r) of 0.9993, accuracy (% recovery) of 103.08%, precision (%RSD) of 0.579%, LOD of 0.6583 ppm, and LOQ of 2.1944 ppm. Based on these results, the ethanol extract of purple yam (Dioscorea alata L.) demonstrated very strong antioxidant activity, and the DPPH method using UV-Visible spectrophotometry fulfilled the established validation parameters. Further research is recommended to isolate, purify, and identify the specific active compounds, particularly anthocyanins and flavonoids, that contribute to the antioxidant activity of purple yam. The use of advanced analytical instruments, such as High-Performance Liquid Chromatography (HPLC) or Liquid Chromatography-Mass Spectrometry (LC-MS), is also recommended to obtain more comprehensive information regarding the active compounds. In addition, the utilization of purple yam as an alternative local food source containing natural antioxidants can be further developed. Keywords: Purple Yam, Dioscorea alata L., Antioxidant, 1,1-Diphenyl-2-picrylhydrazyl, UV-Visible Spectrophotometry, IC₅₀. | 1 |
| Item Type | Skripsi | |
| Keywords | Ubi Uwi Ungu (Purple Yam), Dioscorea alata L., Antioksidan, DPPH, Spektrofotometri UV-Vis, IC₅₀. | 1 |
| Collection | Fakultas Farmasi, S1-Farmasi | |
| Publisher | INSTITUT KESEHATAN DELI HUSADA DELI TUA | |
| Submitted Date | 13-10-2026 | |
| Updated Date | 13-10-2026 | |
| URI | No URI | |
| File |
2218147_PUTRITARINANDASITANGGANG_SKRIPSI-S1.pdf
Login untuk Preview
|
|