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| Advisor(s) | Sembiring, Bunga Mari | |
| Author(s) | SITEPU, MIKAEL FELIK | |
| Issued Date | 2026 | |
| Abstract | Kirinyuh (Chromolaena odorata) leaves are invasive plants with significant potential as traditional medicine due to their secondary metabolite content, particularly flavonoids such as quercetin. This study aimed to identify and determine the quercetin content in the ethanol extract of Kirinyuh leaves using High Performance Liquid Chromatography (HPLC) and validate the analytical method. The simplisia powder was extracted by maceration using 96% ethanol, yielding a 13.25% extract recovery. Simplisia characterization showed a moisture content of 6.16%, water-soluble extractable matter of 29.3%, ethanol-soluble extractable matter of 11.66%, total ash of 4.83%, and acid-insoluble ash of 0.61%, all complying with the Indonesian Herbal Pharmacopoeia standards. Qualitative analysis using Thin Layer Chromatography (TLC) confirmed the presence of quercetin with an Rf value of 0.75, close to the standard quercetin (0.72). Quantitative determination was carried out using an HPLC system with a C18 column, methanol : water (60:40 v/v) mobile phase, flow rate of 1.2 mL/min, and detection wavelength at 370 nm. Method validation provided a linear regression equation of y = 27165x - 111424 with R² = 0.9947, precision (%RSD) of 0.32%, and accuracy (%recovery) ranging from 92.10% to 99.79%, meeting all acceptance criteria. The LOD and LOQ values obtained were 7.99 ppm and 26.65 ppm, respectively. Quantitative analysis revealed that the ethanol extract of Kirinyuh leaves contained an average quercetin content of 13.95 ppm. In conclusion, the developed HPLC method is valid, selective, and sensitive for determining quercetin content in Kirinyuh leaves. | 1 |
| Item Type | Skripsi | |
| Keywords | Chromolaena odorata (Chromolaena odorata), Kuersetin (Quercetin), Ekstraksi (Extraction), HPLC (HPLC), Validasi Metode (Method Validation) | 1 |
| Collection | Fakultas Farmasi, S1-Farmasi | |
| Publisher | INSTITUT KESEHATAN DELI HUSADA DELI TUA | |
| Submitted Date | 28-08-2026 | |
| Updated Date | 29-09-2026 | |
| URI | No URI | |
| File |
2218114_MIKAELFELIKSITEPU_SKRIPSI_S1.pdf
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